The purpose of this study was to investigate EMG fluctuation of antagonist muscle during agonist muscle activation after isotonic strength training. Thirty sedentary males (age 21?1.83 years, Height = 173.90 ? 3.94cm, weight = 73.15 ? 7.51kg) without and history of injuries of shoulder and elbow joints, tendons and muscles involved in elbow flexion movement participated in this study. The subjects were randomly assigned to strength weight training (n=15) and control (n = 15) groups. Then the electromyography (EMG) data (Integrate EMG) from the agonist (Biceps brachii) and antagonist (Triceps brachii) muscles, arm circumference and maximal voluntary contraction (MVC) of the dominant arm were measured in the pre – test. The experimental group performed isotonic strength training of the elbow flexors for 8 weeks 3 times per week. During this period, the control group did not perform any specific types of exercise. At the end of this training period, all parameters were measured again (post – test).